WA-OIG HIGHLIGHTED PUBLICATIONS
August 19th, 2015
Organic and Isotope Geochemistry and Molecular Biology labs:
Sample preparation: Specialised coring, rock grinding and saw facilties for trace organic geochemistry
Sample preparation freeze drying, carbonate removal, centrifugation
Solvent extraction: Soxhlet extraction, ultrasonic extraction, microwave extraction
Molecular sieving techniques for GC-MS and CSIA
Solvent removal: nitrogen evaporator, rotary evaporator
Separation and purification: column chromatography, thin-layer chromatography, silver nitrate chromatography
Hydropyrolysis (HyPy) for generating biomarkers from macromolecular organic matter
Gas chromatography – flame ionisation detector (GC-FID) and gas chromatography – mass spectrometry (GC-MS) analysis: including split/splitless injector, cool on-column injector, flash pyrolysis, microscale sealed-vessel (MSSV) pyrolysis, pyroprobe pyrolysis
Automative preparative GC system for isolation of compounds from complex mixtures
Two-dimensional gas chromatography with time of flight mass spectrometer (GCxGC-TOFMS) performs comprehensive two-dimensional separation of complex mixtures
Compound-specific isotope analysis (CSIA): measurement of δ¹³C, δD, δ15N of liquid and gas samples- these can be from complex mixtures of petroleum, organic extracts containing biomarkers/ biomolecules, microbialites e.g. stromatolites, lake extracts, extracts from sediments or cores, extracts from sponges/ algae/ microbes etc.
Orbitrap liquid chromatography – mass spectrometer (LC-MS) allows high mass resolution, sensitivity and tandem mass spectral structural elucidation of polar compounds, intact lipids, functionalised lipids and metalloporphrins.
Access to δ34S- GC-ICPMS built in collaboration with WA-OIG and UWA for δ34S of organosulfur compounds in complex mixtures.
Laser Pyrolysis- GCMS, Laser Pyrolysis- CSIA.
GC-MSD volatiles (Gas Chromatograph Mass Selective Detectors [Agilent]) Application: GC-MSD with robotic specialised sample preparation systems (solid phase micro extraction – SPME stir bar sorptive extraction – SBSE, purge and trap thermal desorption – PT/TD and closed loop stripping analysis-CLSA units) for analysis of volatile organic and semi-volatile species in various matrices.
Thermal Desorption Unit ‘Unity2’ (TD-unit) [Supplier: Markes] Application: Analysis of VOCs in air samples by adsorbing these compounds onto a resin. This resin is placed into a thermal desorption tube and is then attached to a TD-unit to release the analyte. The unit can also be linked to a GC-irMS for the ¹³C/¹²C and D/H measurements of VOCs.
Robotic SPE (Solid Phase Extraction)
Robotic SPME (Solid Phase Micro Extraction)
SBSE (Stir Bar Sorptive Extraction)
Extraction of extracellular vs. intracellular DNA from environmental samples (water, rock, sediments, soils, stalagmites, corals)
Enzymatic ancient DNA repair
Agarose and denaturing gel electrophoresis (DGGE)
DNA and RNA quantification
Quantitative polymerase chain reaction (qPCR)
Sample preparation for Illumina MiSeq sequencing of PCR products (past and present diversity studies)
Sample preparation for Illumina HiSeq sequencing of environmental metagenomes to provide a holistic overview of the genetic potential now and in the past.
Sample preparation for Illumina HiSeq sequencing of reverse transcribed metatranscriptomes (to study gene expression: who was doing what at the time of sampling)
Bioinformatics and statistical tools used in diversity studies, metagenomics and metatranscriptomics to study ecosystem-environment interactions.
Ectoenzyme activity experiments using fluorescently labelled substrates to study potential rates of microbial decomposition of complex organic matter.
We research the application of biomarkers (organic geochemistry), compound-specific isotope analysis, palaeogenomics and geomicrobiology to a variety of priority areas. These include:
Bldg 500, Curtin University
Freezers incl. a -86C freezer for long-term storage of nucleic acids.
Ancient molecules entombed in rock: ARC Archway Magasine Feature article
Publications related to this article.
1. Grice K, Cao C, Love GD, Bottcher ME, Twitchett R, Grosjean E, Summons R, Turgeon S, Dunning WJ, Jin Y, 2005. Photic Zone Euxinia During the Permian-Triassic Superanoxic Event. Science. 307,706-709.
2. B. Nabbefeld, K. Grice, R.J.Twitchett, R.E. Summons, L. Hays, M.E. Böttcher, A. Muhammad, 2010. An integrated biomarker, isotopic and palaeoenvironmental study through the Late Permian event at Lusitaniadalen. Spitsbergen Earth and Planetary Science Letters. 291, 84-96.
3. Melendez, I., Grice, K., Schwark, L., 2013. Exceptional preservation of palaeozoic steroids in a diagenetic continuum. Nature Scientific Reports. 3.
4. Melendez, I., Grice, K., Trinajstic, K., Ladjavardi, M., Thompson, K., Greenwood, P.F., 2013. Biomarkers reveal the role of photic zone euxinia in exceptional fossil preservation: An organic geochemical perspective. Geology. 41, 123-126.
5. Metcalfe, I., Nicholl, R.S., Willink, R., Ladjavardi, M., Grice, K., 2013. Early Triassic (Induan-Olenekian) conodont biostratigraphy, global anoxia, carbon isotope excursions and environmental perturbations: New data from Western Australian Gondwana. Gondwana Research. 23, 1136-1150.
6. Tulipani, S., Grice, K., Greenwood, P.F., Schwark, L., Summons, R.E., Böttcher, M.E., and Foster, C.B., 2015. Molecular proxies as indicators of freshwater incursion-driven salinity stratification. Chemical Geology. 409, 61-68.
7. Tulipani, S., Grice, K., Greenwood, P., Schwark, L., Haines, P.W., Sauer, P.E., Schimmelmann, A., Summons, R.E., Foster, C.B., Böttcher, M.E., Payton, T.D, and Schwark, L., 2015. Changes of palaeoenvironmental conditions recorded in Late Devonian reef systems from the Canning Basin, Western Australia: A biomarker and stable isotope approach. Gondwana Research. in press.